Referencia

Nature doi:10.1038/nature09679. 8 de diciembre 2010

Autores

Sergi Regot*, Javier Macia*, Núria Conde, Kentaro Furukawa, Jimmy Kjellén, Tom Peeters, Stefan Hohmann, Eulàlia de Nadal, Francesc Posas* y Ricard Solé*

Resumen

Hasta la fecha el campo de la Biología sintética ha intentado diseñar e implementar circuitos, en diferentes organismos, a partir de conceptos básicos de electrónica. Esta aproximación conlleva una gran dificultad a la hora de conectar los circuitos en un entorno biológico. Este trabajo resuelve este problema gracias a una nueva aproximación que consiste en distribuir la computación en diferentes células, que se conectan entre sí con un número mínimo de moléculas señalizadoras. La pequeña biblioteca de células resultante puede ser combinada de múltiples formas para generar una gran diversidad de circuitos. Gracias a esta aproximación se han conseguido implementar circuitos complejos como un multiplexor o un sumador con acarreo.

Descripción

Ongoing efforts within synthetic and systems biology have been directed towards the building of artificial computational devices using engineered biological units as basic building blocks. Such efforts, inspired in the standard design of electronic circuits, are limited by the difficulties arising from wiring the basic computational units (logic gates) through the appropriate connections, each one to be implemented by a different molecule. Here, we show that there is a logically different form of implementing complex Boolean logic computations that reduces wiring constraints thanks to a redundant distribution of the desired output among engineered cells. A practical implementation is presented using a library of engineered yeast cells, which can be combined in multiple ways. Each construct defines a logic function and combining cells and their connections allow building more complex synthetic devices. As a proof of principle, we have implemented many logic functions by using just a few engineered cells. Of note, small modifications and combination of those cells allowed for implementing more complex circuits such as a multiplexer or a 1-bit adder with carry, showing the great potential for re-utilization of small parts of the circuit. Our results support the approach of using cellular consortia as an efficient way of engineering complex tasks not easily solvable using single-cell implementations.

Imágen artículo Enero

REFERENCIA DEL GRUPO E INVESTIGADOR
Sergi Regot está realizando la tesis doctoral en la Unidad de Señalización celular de la Universidad Pompeu Fabra dirigida por el Dr. Francesc Posas. La investigación del grupo se centra en las vías de señalización por MAPKinasas de la familia Hog1/p38. Este estudio se ha realizado en colaboración con el Laboratorio de sistemas complejos de la misma Universidad Pompeu Fabra dirigido por el Dr. Ricard Solé. La investigación del grupo del Dr Solé se centra en la comprensión de la posible presencia de patrones universales en la organización de sistemas complejos.

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