Referencia

Moriel-Carretero & Aguilera; Molecular Cell 37, 690-701, March 12, 2010

Autores

María Moriel-Carretero* y Andrés Aguilera* *Centro Andaluz de Biología Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla-CSIC, Sevilla, Spain

Resumen

El trabajo explica como actúa una mutación específica en el gen RAD3/XPD implicado en reparación por escisión de nucleótidos (NER), que aparentemente no afecta a la capacidad de las células de reparar daños hechos por la luz UV. Esta mutación es capaz de iniciar NER pero no la concluye, provocando una rotura en la horquilla de replicación cuando las células llegan a la fase S del ciclo celular. Estas roturas serían letales si no fuesen reparadas por la vía de la “recombinación”, que se vuelve esencial en estos mutantes. El trabajo sirve además para demostrar que existen dos vías diferentes de re-iniciación de horquillas de replicación, ambas iniciadas por los factores Rad52 y MRX, pero una dependiente de Rad51 y la otra de Pol32.

Descripción

Imagen artículo Mayo

REFERENCIA DEL GRUPO Y/O INVESTIGADOR
El trabajo del grupo dirigido por Andrés Aguilera en CABIMER se centra en entender las causas y consecuencias de la inestabilidad genómica y su relación con otros procesos biológicos tanto en los modelos S. cerevisiae y C. elegans como en cultivos celulares humanos. Trata de entender los fenómenos de inestabilidad asociados a transcripción y a replicación, el acoplamiento entre la transcripción con el transporte del ARN, y la importancia del ARN en la dinámica de los genomas. El estudio de la base genética y molecular de la enfermedad genética Xeroderma pigmentosum, un paradigma de la relación causa-efecto entre inestabilidad genética y cáncer, constituye una de las líneas específicas de investigación, dentro de la cual se ha desarrollado el trabajo publicado en Molecular Cell.

Para ver el artículo completo, pulse aquí

Did you publish an interesting article recently?

Send it through our application form and we will contact you. Age limit: 32.

The selected articles will participate at the Fisher Scientific Prize which will be given during SEBBM conference, that will take place at Spain (free registration, travel and accommodation).

More articles of the month

Inactivation of Capicua in adult mice causes T-cell lymphoblastic lymphoma

01-12-2017

CIC (also known as Capicua) is a transcriptional repressor negatively regulated by RAS/MAPK signaling. Whereas the functions of Cic have been well characterized in Drosophila, little is known about its...

Read more

Physical proximity of chromatin to nuclear pores prevents harmful R loop accumulation contributing to maintain genome stability

01-11-2017

During transcription, the mRNA may hybridize with DNA, forming an R loop, which can be physiological or pathological, constituting in this case a source of genomic instability. To understand the...

Read more

Whi7 is an unstable cell-cycle repressor of the Start transcriptional program

01-10-2017

Start is the main decision point in eukaryotic cell cycle in which cells commit to a new round of cell division. It involves the irreversible activation of a transcriptional program...

Read more

CTCF orchestrates the germinal centre transcriptional program and prevents premature plasma cell differentiation

01-09-2017

In germinal centres (GC) mature B cells undergo intense proliferation and immunoglobulin gene modification before they differentiate into memory B cells or long-lived plasma cells (PC). GC B-cell-to-PC transition involves...

Read more

Catalytic Cycle of the N-Acetylglucosaminidase NagZ from Pseudomonas aeruginosa

01-08-2017

The N-acetylglucosaminidase NagZ of Pseudomonas aeruginosa catalyzes the first cytoplasmic step in recycling of muropeptides, cell-wall-derived natural products. This reaction regulates gene expression for the β-lactam resistance enzyme, β-lactamase. The...

Read more

Crebbp loss cooperates with Bcl2 overexpression to promote lymphoma in mice.

01-07-2017

CREBBP is targeted by inactivating mutations in follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL). Here, we provide evidence from transgenic mouse models that Crebbp deletion results in deficits...

Read more

Local amplifiers of IL-4Rα-mediated macrophage activation promote repair in lung and liver

01-06-2017

The type 2 immune response controls helminth infection and maintains tissue homeostasis but can lead to allergy and fibrosis if not adequately regulated. We have discovered local tissue-specific amplifiers of...

Read more

Programmed mitophagy is essential for the glycolytic switch during cell differentiation

01-05-2017

Retinal ganglion cells (RGCs) are the sole projecting neurons of the retina and their axons form the optic nerve. Here, we show that embryogenesis-associated mouse RGC differentiation depends on mitophagy...

Read more

Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes

01-04-2017

The Bcl-2 (B-cell lymphoma 2) protein Bax (Bcl-2 associated X, apoptosis regulator) can commit cells to apoptosis via outer mitochondrial membrane permeabilization. Bax activity is controlled in healthy cells by...

Read more

Histone chaperone activity of Arabidopsis thaliana NRP1 is blocked by cytochrome c

01-03-2017

Higher-order plants and mammals use similar mechanisms to repair and tolerate oxidative DNA damage. Most studies on the DNA repair process have focused on yeast and mammals, in which histone...

Read more

Protector Members