Reference

naturensp 420Nat Commun. 2016 May 6;7:11431. doi: 10.1038/ncomms11431.

Authors

Guiomar Martín, Pablo Leivar, Dolores Ludevid, James M. Tepperman, Peter H. Quail y Elena Monte.

Abstract

Plastid-to-nucleus retrograde signals emitted by dysfunctional chloroplasts impact photomorphogenic development, but the molecular link between retrograde- and photosensory-receptor signalling has remained unclear. Here, we show that the phytochrome and retrograde signalling (RS) pathways converge antagonistically to regulate the expression of the nuclear-encoded transcription factor GLK1, a key regulator of a light-induced transcriptional network central to photomorphogenesis. GLK1 gene transcription is directly repressed by PHYTOCHROME-INTERACTING FACTOR (PIF)-class bHLH transcription factors in darkness, but light-activated phytochrome reverses this activity, thereby inducing expression. Conversely, we show that retrograde signals repress this induction by a mechanism independent of PIF mediation. Collectively, our data indicate that light at moderate levels acts through the plant's nuclear-localized sensory-photoreceptor system to induce appropriate photomorphogenic development, but at excessive levels, sensed through the separate plastid-localized RS system, acts to suppress such development, thus providing a mechanism for protection against photo-oxidative damage by minimizing the tissue exposure to deleterious radiation.

Description

En plantas, los cloroplastos envían señales al núcleo para informarles de las condiciones ambientales que las rodean (señalización retrógrada). Esta vía de señalización se activa cuando las plantas crecen en condiciones ambientales que producen defectos en el funcionamiento de los cloroplastos, como por ejemplo, cuando hay excesiva luz solar. Mediante la señalización retrógrada, los cloroplastos son capaces de regular la expresión de genes nucleares y adaptar su propia capacidad fotosintética. En este trabajo hemos descubierto que la señalización que ocurre de cloroplasto a núcleo no solo regula el funcionamiento de los cloroplastos, sino que además permite a las plantas adaptar su desarrollo global. Las señales retrógradas reprimen transcripcionalmente GLK1, un inductor del desarrollo fotomorfogénico que se activa cuando las plantas crecen en condiciones de luz óptimas. Así pues, mediante la regulación transcripcional antagonística de GLK1, las vías de señalización retrógrada y lumínica ajustan el desarrollo fotomorfogénico a la cantidad de luz que reciben las plantas, y a la capacidad que tienen los cloroplastos de captarla y procesarla.

 

Grupo investigador

 

REFERENCIA DEL GRUPO INVESTIGADOR

El grupo de la Dra. Elena Monte forma parte del Programa de Desarrollo y Transducción de Señales, situado en el Centro de Investigación en Agrigenómica (CRAG) CSIC-IRTA-UAB en Barcelona. Su investigación se centra en entender cómo las plantas utilizan la luz no como fuente de energía, sino como fuente de información. Este proceso es esencial, ya que las plantas, al ser organismos sésiles, necesitan informarse de los cambios que se producen en las condiciones de luz ambientales (como día/noche, sombra o intensidad de luz) para ajustar su desarrollo en respuesta a estos cambios y adaptarse para sobrevivir.

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