Reference

Nat Commun. 2020 Jan 9;11(1):156. doi: 10.1038/s41467-019-13990-wPortada Marzo 2020

Abstract

Technical problems intrinsic to the purification of preribosome intermediates have limited our understanding of ribosome biosynthesis in humans. Addressing this issue is important given the implication of this biological process in human disease. Here we report a preribosome purification and tagging strategy that overcomes some of the existing technical difficulties. Using these tools, we find that the pre-40S precursors go through two distinct maturation phases inside the nucleolus and follow a regulatory step that precedes late maturation in the cytoplasm. This regulatory step entails the intertwined actions of both PARN (a metazoan-specific ribonuclease) and RRP12 (a phylogenetically conserved 40S biogenesis factor that has acquired additional functional features in higher eukaryotes). Together, these results demonstrate the usefulness of this purification method for the dissection of ribosome biogenesis in human cells. They also identify distinct maturation stages and metazoan-specific regulatory mechanisms involved in the generation of the human 40S ribosomal subunit.

Description

Este artículo describe un nuevo método que permite visualizar, purificar y caracterizar distintos intermediarios de formación de los ribosomas en células humanas. Utilizando este método, los autores consiguen definir las funciones de varios factores esenciales para el ensamblaje de la subunidad ribosómica pequeña. Además, descubren un paso de maduración de esta subunidad que está mediado por una ribonucleasa y que es específico de células humanas.

 

Grupo

 

REFERENCIA DEL GRUPO INVESTIGADOR

Mercedes Dosil dirige un grupo en el Centro de Investigación del Cáncer de Salamanca dedicado al estudio del ensamblaje de los ribosomas. Sus investigaciones combinan experimentos de purificación y análisis composicional de pre-ribosomas en distintos pasos de maduración, con estudios funcionales de factores esenciales para esos pasos utilizando técnicas genéticas, bioquímicas y de microscopía de alta resolución. Sus líneas actuales de trabajo se centran en eventos necesarios para el ensamblaje de la subunidad ribosómica pequeña que están alterados en células tumorales y en algunas ribosomopatías, un grupo de enfermedades congénitas causadas por defectos en la formación de los ribosomas.

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