Referencia

Molecular cell 53, no. 4 (2014): 549-561.

Autores

Mariona Nadal-Ribelles (1,3), Carme Solé (1,3), Zhenyu Xu (2), Lars M. Steinmetz (2), Eulàlia de Nadal (1,4) and Francesc Posas (1,4) (1) Cell Signaling Unit, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), E-08003 Barcelona, Spain (2) EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany (3) These authors contributed equally to this manuscript. (4) Corresponding authors: This email address is being protected from spambots. You need JavaScript enabled to view it.; This email address is being protected from spambots. You need JavaScript enabled to view it.

Resumen

Genomic analysis has revealed the existence of a large number of long non-coding RNAs (lncRNAs) with different functions in a variety of organisms, including yeast. Cells display dramatic changes of gene expression upon environmental changes. Upon osmostress, hundreds of stress-responsive genes are induced by the stress-activated protein kinase (SAPK) p38/Hog1. Using whole-genome tiling arrays, we found that Hog1 induces a set of lncRNAs upon stress. One of the genes expressing a Hog1-dependent lncRNAs in antisense orientation is CDC28, the CDK1 kinase that controls the cell cycle in yeast. Cdc28 lncRNA mediates the establishment of gene looping and the relocalization of Hog1 and RSC from the 3’UTR to the +1 nucleosome to induce CDC28 expression. The increase in the levels of Cdc28 results in cells able to re-enter the cell cycle more efficiently after stress. This may represent a general mechanism to prime expression of genes needed after stresses are alleviated.

Descripción

En respuesta a estímulos extracelulares las quinasas de respuesta a estrés (SAPK) modulan la expresión génica para maximizar la supervivencia celular. En levadura, la SAPK Hog1 es responsable de coordinar esta reprogramación transcripcional en respuesta a estrés osmótico. En este trabajo se describe el rol de la MAPK controlando la transcripción de RNAs no codificantes (lncRNAs) en dirección antisentido y su relevancia fisiológica. En respuesta a estrés, la inducción de lncRNAs junto con la presencia de Hog1 crea una estructura génica en forma de bucle (“gene looping”) que enlaza terminador con la zona del nucleosoma +1 promoviendo la remodelación de éste y el consiguiente incremento de la transcripción del gen. Esto permite a las células restablecer el ciclo celular para su adaptación después del estrés.

grupo

REFERENCIA DEL GRUPO INVESTIGADOR

Mariona Nadal-Ribelles ha realizado la tesis doctoral en el Grupo de Señalización Celular de la Universidad Pompeu Fabra dirigido por el Dr. Francesc Posas y Dra. Eulàlia de Nadal. La investigación del grupo se centra en el estudio de la vía de señalización en respuesta a estrés controlada por las MAPK de la familia de Hog1/p38, y los mecanismos moleculares que permiten una respuesta adaptativa al estrés como la regulación del ciclo celular y la transcripción.

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