16.09.2020  STATUS
Virtual - on line

Date: Wednesday, September 16, 2020
Time: 05:30 PM Central European Summer Time
Duration: 1 hour

CRISPR/Cas9 technology has revolutionized genome editing by offering a simple method to tag proteins at endogenous loci, facilitating the study of protein biology while maintaining proper transcriptional regulation, expression levels and stoichiometry with binding partners. By contrast, ectopic expression of tagged proteins can lead to a
variety of overexpression artifacts, like mislocalization, aggregation, or dysregulation of degradation. HiBiT, an 11-amino-acid bioluminescent peptide, represents an ideal tag for
endogenous labeling due to its small size and large, linear dynamic range. In this live talk, we will highlight an efficient, cloning-free method for knock-in of HiBiT. We will demonstrate that this represents a scalable strategy for studying protein dynamics, including abundance, localization, modification, and interactions. We will also focus on the application of this method to monitoring PROTAC-induced protein degradation, highlighting the importance of endogenous expression in achieving biologically meaningful results.

This webinar is organised by Promega and the SEBBM.

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